Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (FITC)
Referência M1202-78-100ug
Tamanho : 100ug
Marca : US Biological
Contactar o distribuidor local :
Telefone : +1 850 650 7790
M1202-78 Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (FITC)
Clone Type
MonoclonalHost
mouseSource
humanSwiss Prot
P06702Isotype
IgG1Grade
Affinity PurifiedApplications
FCCrossreactivity
Bo Ca Eq Fe Gt Gp Hu Mk Po Rb RtShipping Temp
Blue IceStorage Temp
-20°CL1 Protein or Calprotectin Molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required.
Optimal working dilutions to be determined by researcher.
Recommended Negative Control:
I1904-78R3: IgG1 Murine Negative Control (FITC)
Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2617 (MAC387)|Host: mouse|Source: human|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Human monocytes|Specificity: Recognizes human L1 Protein or Calprotectin Molecule. Variable results have been reported for the staining of brain macrophages and microglia. Species Crossreactivity: equine, Cynomolgus monkey, Rhesus monkey, bovine, baboon, rabbit, canine, feline, porcine, guinea pig, goat, rat, rabbit, Fallow deer, Pygmy hippopotamus, mink and Marmoset monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human monocytes
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human L1 Protein or Calprotectin Molecule. Variable results have been reported for the staining of brain macrophages and microglia. Species Crossreactivity: equine, Cynomolgus monkey, Rhesus monkey, bovine, baboon, rabbit, canine, feline, porcine, guinea pig, goat, rat, rabbit, Fallow deer, Pygmy hippopotamus, mink and Marmoset monkey