Growth Associated Protein (GAP)-43/b-50

GAP-43 is a protein expressed at elevated levels by developing or regenerating neurons during axon growth. While this neuron-specific membrane protein is a component of axonal growth cones, it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 0.1-0.5ug/ml on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X- 100 to incubation buffer will increase cellular permeability. Western Blot: will detect a single 43-48kD band in western blots of membrane fractions of growing neurons.
Immunoprecipitation: 5-10ug/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.