mouse Anti-Cytokeratin 18 (FITC) Monoclonal Antibody [Clone:5F227]

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Referência C9097-23D3-250ul

Tamanho : 250ul

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Telefone : +1 850 650 7790

Marca : US Biological

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

C9097-23D3 Cytokeratin 18 (FITC)

Clone Type

Monoclonal

Host

mouse

Source

human

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IC IHC

Crossreactivity

Bo Ca Hm Hu Mo Po Rt Sh

Shipping Temp

Blue Ice

Storage Temp

-20°C

C9097-23D3 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen, Paraffin-embedded): 1:10; 1hr at 37°C.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 5F227|Host: mouse|Source: human|Concentration: Not Determined |Form: Supplied as a liquid, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). |Purity: Purified by Protein A affinity chromatography.|Immunogen: Human cytokeratin 18 from HeLa cytoskeletal preparation.|Specificity: Recognizes Cytokeratin 18 (MW: 45kD) of all human simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with cultured HeLa and MCF-7 cells. |Species Crossreactivity: mouse, rat, bovine, porcine, canine, hamster, sheep and fish (trout).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human cytokeratin 18 from HeLa cytoskeletal preparation.

Form

Supplied as a liquid, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes Cytokeratin 18 (MW: 45kD) of all human simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with cultured HeLa and MCF-7 cells. |Species Crossreactivity: mouse, rat, bovine, porcine, canine, hamster, sheep and fish (trout).

References

1. Bartek, J., et al., J. Pathol. 164: 215-224 (1991). 2. Lanerova, L., et al., Hybridoma 7: 495-504 (1988). 3. Markl, J., et al., Eur. J. Cell Biol. 50: 1-16 (1989). 4. Moll, R., et al., Cell 31: 11-24 (1982). 5. Gomi, H., et al., Neuron 14: 29-41 (1995). 6. Bantel, H., et al., Eur. J. Cell Biol. 80: 230-239 (2001). 7. Stumptner, C., et al., Hepatology 35: 1053-1062 (2002). 8. Ikenouchi, J., et al., J. Cell. Sci. 116: 1959-1967 (2003).