Mouse Anti-CA12 Monoclonal Antibody [Clone:CL0280]

Referência AMAb90639

Tamanho : 100ul

Marca : Atlas Antibodies

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AMAb90639
carbonic anhydrase XII

Anti-CA12 Antibody

Monoclonal Antibody against HUMAN CA12

0.7 mg/ml
info@quimigen.pt

Immunohistochemical staining of human kidney shows strong membranous and/or moderate cytoplasmic immunoreactivity in subsets of renal tubules, while glomeruli are negative.

Product details

  • Target protein: carbonic anhydrase XII
  • Target gene: CA12
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000032373 (88%)RAT - ENSRNOG00000017766 (88%)
  • Clonality: Monoclonal
  • Clone ID: CL0280
  • Isotype: IgG1
  • Host: Mouse
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-02614
  • Concentration: 0.7 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml

Validation

  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: carbonic anhydrase XII
  • Gene name: CA12
  • Alternative gene names: HsT18816, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Vergara D, Ravaioli S, Fonzi E, Adamo L, Damato M, Bravaccini S, Pirini F, Gaballo A, Barbano R, Pasculli B, Franck J, Fournier I, Salzet M, Maffia MInt J Mol Sci , 2020 Jan 22; 21(3):715. Epub 2020 Jan 222020 Jan 22

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas