Mouse Anti-ATRX Monoclonal Antibody [Clone:CL0537]

Referência AMAb90784

Tamanho : 100ul

Marca : Atlas Antibodies

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AMAb90784
alpha thalassemia/mental retardation syndrome X-linked

Anti-ATRX Antibody

Monoclonal Antibody against HUMAN ATRX

1.0 mg/ml
info@quimigen.pt

Product details

  • Target protein: alpha thalassemia/mental retardation syndrome X-linked
  • Target gene: ATRX
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000031229 (96%)RAT - ENSRNOG00000002550 (97%)
  • Clonality: Monoclonal
  • Clone ID: CL0537
  • Isotype: IgG1
  • Host: Mouse
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-04233
  • Concentration: 1.0 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 2-10 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Genetic validation in WB by siRNA knockdown. 

Target information

  • Protein name: alpha thalassemia/mental retardation syndrome X-linked
  • Gene name: ATRX
  • Alternative gene names: JMS, RAD54, XH2, XNP, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas