Mouse 43 kDa receptor-associated protein of the synapse (RAPSN) ELISA Kit
Referência AE24378MO-480
Tamanho : 5x96tests
Marca : Abebio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Mouse (Mus musculus) |
| UniProt | P12672 |
| Abbreviation | RAPSN |
| Alternative Names | CMS1D; CMS1E; MGC3597; RNF205; 43 kD receptor-associated protein of the synapse|43 kda postsynaptic protein|acetylcholine receptor-associated 43 kda protein|rapsyn|receptor-associated protein of the |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1 - 2 0 0 μ L |
| Detection Wavelengt | 450 nm |
Test principle
This assay employs a two-site sandwich ELISA to quantitate RAPSN in samples. An antibody specific for RAPSN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAPSN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAPSN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPSN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview
Components
Reagents | Quantity | Reagents | Quantity |