Monoglyceride lipase (1-313, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP
Tag	His-Tag
Predicted MW	36.4 kDa (333aa) confirmed by MALDI-TOF
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 85% by SDS-PAGE
Presentation State	Purified
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Preparation	Liquid purified protein
Recommended Dilution	SDS-PAGE
Protein Description	Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Shipping	Blue Ice
Reference Data
RefSeq	NP_009214
Locus ID	11343
UniProt ID	Q99685
Cytogenetics	3q21.3
Synonyms	Monoglyceride lipase, Hu-K5, HuK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase
Summary	This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Feb 2012
Protein Categories	Intracellular Proteins
Protein Families	Druggable Genome, Protease
Protein Pathways	Glycerolipid metabolism, Metabolic pathways

FAQs

Protein FAQs

SDS

SDS for AR09706PU-N

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein