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> Monkey Interleukin 6,IL-6 ELISA Kit

Monkey Interleukin 6,IL-6 ELISA Kit

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Referência CSB-E10050Mo-IS-24T

Tamanho : 24T

Marca : Cusabio

Contactar o distribuidor local :


Telefone : +1 850 650 7790

CSB-E10050Mo-IS
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 6 (interferon, beta 2)
Associated names
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation
Uniprot No.
Species
Monkey
Sample Types
cell culture supernates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
15.625 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Cell Culture Supernates (n=4)

1:1

Average %

89

Range %

84-97

1:2

Average %

92

Range %

85-96

1:4

Average %

97

Range %

90-103

1:8

Average %

91

Range %

86-98

Recovery

The recovery of monkey IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Cell Culture Supernates (n=5)

94

87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

3.372

3.405

3.389

3.285

1000

1.857

1.932

1.895

1.791

500

0.771

0.765

0.768

0.665

250

0.419

0.421

0.420

0.316

125

0.251

0.243

0.247

0.144

62.5

0.169

0.162

0.166

0.062

31.25

0.118

0.121

0.119

0.016

0

0.101

0.106

0.103

　

ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This ELISA kit provides quantitative measurement of IL-6 in monkey samples, supporting immunological research on this pleiotropic cytokine central to acute-phase responses, inflammation, and immune cell differentiation. Because IL-6 is often studied in stimulated primate cell systems where secretion kinetics and magnitude vary dramatically by stimulus and cell type, this kit is designed to address those experimental demands directly. Detection Range: The 31.25–2000 pg/mL range captures IL-6 concentrations typically observed in both unstimulated and mitogen- or LPS-stimulated monkey PBMC or...

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Target Background

Function
(From Uniprot)
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the...
Subcellular Location
Secreted.
Protein Families
IL-6 superfamily
Database Links

STRING:

UniGene: