SM1855F | MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID

MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

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SM1855F

MHC Class I (monomorphic) mouse monoclonal antibody, clone 2G5, FITC

<strong>Published customer image:</strong> <br>Mouse anti Mouse MHC Class I antibody, clone 2G5 (<strong>MCA2189</strong>) used for the evaluation of MHC class I expression on Guinea Pig lung infiltrates in a mycobacterial disease model by flow cytometry.<br><strong>Image caption:</strong><br>Evaluation of neutrophil infiltration and MHC expressions in the infected lung. The expression of MHC I, MHC II and percentage of neutrophils were monitored at different time points during the course of therapy. The bar diagram shows the mean percentage of MHC expression on gated population or neutrophil content in the lungs of drug or drug plus MIP group. Data represents the mean value of four animals in each group. * p<0.05. Entire lymphocyte negative region was gated based on forward versus side scatter and MHC expression or neutrophil infiltration was analyzed on the gated region. The representative plots show the MHC expression or neutrophil infiltration in different experimental groups along with the suitable negative and positive controls. Percentage value has been mentioned in the assigned region in each plot.<br><br>From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, Katoch V, <em>et al</em>. (2012)<br>Efficacy of <em>Mycobacterium indicus pranii</em> Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.<br><strong><a href="https://www.ncbi.nlm.nih.gov/pubmed/22844392"target="_blank" rel="noopener">PLoS ONE 7(7): e39215</a></strong>.<br>This image is from an open access article distributed under the terms of the <a href="https://creativecommons.org/licenses/by/4.0/"target="_blank" rel="noopener">Creative Commons Attribution License</a>.

Specifications
Product Data
Clone Name	2G5
Application	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of Neat-1/5 diluted antibody to label 10e6 cells in 100 µl.
Reactivity	Bovine, Guinea Pig, Hamster, Human, Mouse, Porcine, Rat
Antibody Host	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Purified H-2Kb and H-2Db MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
Specificity	This antibody recognises a monomorphic epitope on the MHC class I antigen. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some Canine samples suggesting that this antibody may recognise a polymorphic epitope of Canine MHC class 1.
Buffer	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Shipping	Blue Ice
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Synonyms	MHC Class 1, HLA Class 1
Reference Data

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for SM1855F

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

Referência SM1855F

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MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

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MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

Referência SM1855F

Contactar o distribuidor local :

MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

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