MCP-3 ELISA Kit (Mouse)
Referência OKAG00092
Tamanho : 1plate
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
MCP-3 ELISA Kit (Mouse) : 96 Wells (OKAG00092)
| Datasheets/Manuals | Printable datasheet for MCP-3 ELISA Kit (Mouse) : 96 Wells (OKAG00092) |
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| COA Datasheet | Click Here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Product Format | 1 x 96-Well Plate or 12 x 8-Well Strips | ||||||||||||||||||||||
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | 4.5 hours | ||||||||||||||||||||||
| ELISA Kit Principle | The Aviva Murine MCP-3ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mMCP-3concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theMurineMCP-3cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. | ||||||||||||||||||||||
| ELISA Kit Range | 8-2000 pg/mL | ||||||||||||||||||||||
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| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Cell lysates, sera and plasma | ||||||||||||||||||||||
| Sensitivity | 8 pg/mL | ||||||||||||||||||||||
| Specificity | The Murine MCP-3 ELISA is capable of recognizing both recombinant and naturally produced Murine MCP-3 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity. • Murine: Eotaxin The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human: MCP-1, MCP-2, MCP-3, MCP-4 • Murine: C-10, CTACK, Eotaxin-2, Exodus-2, JE, MCP-2, MCP-5, MDC, MIP-1α, MIP-1β, MIP-1γ, MIP-3α, MIP-3β, RANTES • Rat: MCP-1 | ||||||||||||||||||||||
| Assay Info | Quantitative Colorimentric Sandwich ELISA |
| Gene Symbol | Ccl7 |
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| Gene Full Name | chemokine (C-C motif) ligand 7 |
| Alias Symbols | C-C motif chemokine 7, fi, fic, intercrine/chemokine MARC, ma, marc, mcp, MCP-, MCP-3, mcp3, monocyte chemoattractant protein 3, monocyte chemotactic protein 3, Protein FIC, RANTES/sis homolog, Scy, Scya7, small-inducible cytokine A7. |
| NCBI Gene Id | 20306 |
| Protein Name | C-C motif chemokine 7 |
| Description of Target | Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity). |
| Uniprot ID | Q03366 |
| Protein Accession # | NP_038682.1 |
| Nucleotide Accession # | NM_013654.3 |
