Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.
 
> Maf1 (NM_026859) Mouse Untagged Clone

Maf1 (NM_026859) Mouse Untagged Clone

Imprimir Imprimir
Please log in

Referência MC201311

Tamanho : 10ug

Contactar o distribuidor local :


Telefone :

Maf1 (NM_026859) Mouse Untagged Clone

Be the first to review this product
SKU
MC201311
Maf1 (untagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2, (10ug)
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
 

Other products for "Maf1 or NM_026859"

Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Maf1
Synonyms 1110068E11Rik; AU042856
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC016260 sequence for NM_026859
GGGGTTGGGCCCGTCGGACGCTTGTTGTTGTCGGGTCGGGGGAGGCGGAGGTCGCTGTTTCGTTCTCTGG CTGGTACGATCTCGGCGGTCCGCGGCAGGTTGGTCGCGAAAGCGGGCGTTGCAGGGGGGCGAGGCTATGT CGCGGCTGCAGCCCGTATGGGCCGGCAGGGCCTGGAGTAACGGGACGTCGCCGCGAAGCTTCTTCCCCCG GATACAGTGCGGCCCGAGTGGAGGCCGCGGCGCCGCCCTCCAGTCCTGAGGAACCCGCGCTGCGCAAAGC CCGCGCCGCCCTCACCTCGGCACCGACACTAAACAACCCGTCTACCCTGGCTTGATCTAGCCCAATTCTG GCAGGTAGTGCTGCCCTAATCCCACTGGCACAAGAATCTCCCTTCCCAAAGGACATGAAGCTATTGGAGA ACTCCAGCTTTGAGGCCATCAACTCACAGTTGACAGTGGAGACTGGAGATGCCCATATTATTGGCAGGAT TGAAAGCTACTCGTGTAAGATGGCGGGAGATGATAAACATATGTTCAAGCAGTTCTGCCAGGAGGGCCAG CCCCATGTGTTGGAGGCACTGTCCCCACCCCAAACTTCAGGCCTCAGTCCCAGCAGACTGAGCAAGAGCC AGGGTGGTGATGATGAGAGTCCTCTGAGCGACAAGTGCAGCCGCAAGACCCTCTTCTATCTGATTGCCAC CCTCAATGAGTCCTTCCGGCCAGACTATGACTTCAGCACAGCCAGAAGTCATGAATTCAGCCGAGAGCCA AGCCTCCGCTGGGTGGTAAATGCAGTCAACTGCAGCCTGTTTTCAGCTGTTCGTGAAGACTTCAAGGCCC TGAAGCCACAGCTGTGGAATGCAGTGGATGAGGAGATCTGCTTAGCTGAGTGTGACATCTACAGCTATAA CCCAGATCTAGACTCCGACCCCTTTGGGGAAGATGGAAGCCTCTGGTCATTCAACTATTTCTTTTACAAT AAGAGACTGAAGCGAATTGTCTTCTTTAGCTGCCGCTCCATCAGTGGCTCCACGTACACACCCTCAGAGG CAGGCAATGCACTGGACTTGGAACTGGGGGCAGAGGAGGCTGATGAAGAGAGTGGAGGTGGAGGCGGTGA GGGCAGGGCAGAGGAGACCAGCACCATGGAGGAAGACAGGGTTCCAGTGATCTGTATGTGATGAGAAGAA GCAGAGGTCTCAGCGTCATCCAGCTCTGACCAATCCCTGGACCTGTCTACCTGAGGAGCTCCAGAGCTTC CCCAGATGCTGGCTATGCCTTGGCACTGACACCGTCCTGGCGCTGCCCAAGGCTATACCTGTGTAGCCCT TTGGATTCAGCCTGTGGATGTCCACTCATCCCTGCAGGCTCCTACTGCCCATGCTGTGGCTGGATTAAAC AGCACGGGGTCTGATAGAAAACGGGACTGCTCTGCACCAACAGCCTGTCACAGACAGGACAGCTGGACCA CAGAGTTTATTTTTGTATTTCTTATTGGGCCTGTACACTCCAGCTCTAAGGGCCAGTAGTTTGAAGCCCT ATAAGTGGTGCCCAGTGTTCATGGGGTCTGGTCTTTGACCGGCCTCTGGTGCCCACTTTTGTCTCCCACT TTACCAGGTGGGCCACATGCACTGAGTGTCACTTTGCTGCAGCTCATTTGTTTCCAATAAAAGTTTCTGT GACTTAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_026859
Insert Size 777 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016260, AAH16260
RefSeq Size 1702 bp
RefSeq ORF 777 bp
Locus ID 68877
UniProt ID Q9D0U6
Cytogenetics 15 D3
Summary Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (By similarity). Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (PubMed:30110641). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3, encode the same protein.
SKU Description Size
MG203339 Maf1 (tGFP-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1) 10 ug
MR203339 Maf1 (Myc-DDK-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2 10 ug
MR203339L3 Lenti ORF clone of Maf1 (Myc-DDK-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2 10 ug
MR203339L4 Lenti ORF clone of Maf1 (mGFP-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2 10 ug

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Você também pode estar interessado nos seguintes produtos:



Referência
Descrição
Cond.
Price Bef. VAT
HY-13018-10mg
MRT67307 [1190378-57-4]
 10mg