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> Lysostaphin [9011-93-2]

Lysostaphin [9011-93-2]

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Referência HY-P2329-1mg

Tamanho : 1mg

Marca : MedChemExpress

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Description

Lysostaphin is an antistaphylococcal agent. Lysostaphin has activities of three enzymes namely, glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase[1].

In Vitro

Lysostaphin (16 μg/mL, 0-260 minutes) exerts its antibacterial effect by hydrolyzing the peptidoglycan cross-linkages of Staphylococcus aureus, weakening the mechanical strength of the cell wall and causing structural damage[3].
Product Information
Molecular weight: 27 kDa
Effective pH range: 5.0-8.0

Instructions
Can be dissolved in ddH2O at a concentration of 1 mg/mL. Fresh preparation is recommended. Or dissolve in buffer according to specific experimental references.

Notes
PCMB, PMSF, DTT, etc. have no obvious effect on enzyme activity at 1 mM. Ba2+, Mn2+, Ca2+, Mg2+ and EDTA show a certain degree of inhibitory effect on enzyme activity at a concentration of 1 mM.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lysostaphin Related Antibodies
In Vivo

Lysostaphin (5 mg/kg, intravenous injection, once daily for 3 consecutive days) effectively clears systemic Staphylococcus aureus (MSSA) infections in CF-1 mice[2].
Lysostaphin (50 mg/kg, intravenous injection, single dose) effectively clears Staphylococcus aureus (MSSA and MRSA) infections from the kidneys, spleen, and liver, but is not superior to repeated lower doses[2].
Lysostaphin (1 mg/kg, intravenous injection, once daily for 3 consecutive days) combined with Oxacillin (HY-B0925A) achieves the same antibacterial effect with a lower concentration of Lysostaphin. When combined with Vancomycin, low-dose Lysostaphin (1 mg/kg) similarly shows enhanced efficacy against MRSA[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Systemic infection model in CF-1 mice induced by methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA)[2]
Dosage: 1, 5, 25, 50 mg/kg
Administration: Intravenous injection (i.v.), single high-dose administration: 25 mg/kg or 50 mg/kg intravenous injection, single dose; Once daily administration: 1 or 5 mg/kg, once daily for 3 days.
Result: Cleared MSSA from the blood and organs of mice (5 mg/kg for 3 days).
Cleared MSSA and MRSA from the kidneys, spleen, and liver (A higher single dose of 50 mg/kg).
Improved effectiveness and allowed reduction of the lysostaphin dose to 1 mg/kg for MRSA infections (Combined with Oxacillin (HY-B0925A) or Vancomycin (HY-B0671)).
Essai clinique
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Lysostaphin]
EC Number

3.4.24.75
Enzyme Activity

≥1200 U/mg soild
Unit Definition

One unit is defined as the amount of enzyme required to reduce the OD620 absorption value by 0.01 per minute at 37°C and pH 7.5

Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvant et solubilité
In Vitro: 

H2O : ≥ 1 mg/mL

*"≥" means soluble, but saturation unknown.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
Références

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HY-P2329-5mg
Lysostaphin [9011-93-2]
 5mg