OKCD02274 | Lysophosphatidic Acid ELISA Kit Quimigen Portugal

Technical Documents

Datasheets/Manuals	Printable datasheet for Lysophosphatidic Acid ELISA Kit (OKCD02274)
COA Datasheet	ClickHere to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

All Species

Application

ELISA-Comp

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

2h

ELISA Kit Linearity

Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
serum(n=5)	90-101%	83-104%	81-98%	91-101%
EDTA plasma(n=5)	93-101%	83-97%	78-98%	95-105%
heparin plasma(n=5)	97-104%	95-103%	91-98%	87-101%

ELISA Kit Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to LPA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled LPA analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of LPA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LPA in the sample.

ELISA Kit Range

123.5-10,000ng/mL

ELISA Kit Recovery

Matrix	Recovery Range (%)	Average (%)
serum(n=5)	95-102	99
EDTA plasma(n=5)	87-99	96
heparin plasma(n=5)	93-105	101

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidic Acid (LPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidic Acid (LPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Anti-Lysophosphatidic Acid Microplate	96 Wells (12 x 8 Well Strips)
Lysophosphatidic Acid Lyophilized Standard	2
100X Lysophosphatidic Acid Biotin Complex	120 uL
100X Avidin/HRP Conjugate	120 uL
Standard Diluent	1 x 20 mL
Biotin Complex Diluent	1 x 12 mL
Avidin/HRP Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Additional Information

CAS: 22002-87-5
PubChem: 5497152

Reconstitution and Storage

-20°C|2°C to 8°C

Sample Type

Serum, plasma and other biological fluids

Sensitivity

52.7 ng/mL

Assay Info

Assay Methodology: Competitive Inhibition Immunoassay

Target Info

Gene Symbol	LPA
Alias Symbols	Lysophosphatidic acid; 2-Hydroxy-3-(phosphonooxy)propyl oleate; (2-hydroxy-3-phosphonooxypropyl) (Z)-octadec-9-enoate
Protein Name	Lysophosphatidic Acid

Protocols & Data

Lysophosphatidic Acid ELISA Kit

Referência OKCD02274

Contactar o distribuidor local :

Marca : Aviva Systems Biology

Contactar o distribuidor local :

Lysophosphatidic Acid ELISA Kit (OKCD02274)

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