LSD1, Fluorescent, BioAssay™ Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)

LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.

Intended Use:
The LSD1 BioAssay™ Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1.

Test Principle:
The key to the LSD1 BioAssay™ Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (lamda ex=530±13 nm, lambda em=590±18 nm) is measured using a plate reader.

Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt

Applications:
Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.

Kit Components:
White microtiter plate, 1x 96 wells
LSD1 recombinant enzyme, 1x30ug
Methylated peptide substrate (200uM) 1x500ul
Amplex Red reagent (10mM) 1x50ul
Peroxidase (10U/ml), 1x100ul
2x LSD1 assay buffer, 1x20ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.