LPA ELISA Kit (Human)
Referência OKEH00633
Tamanho : 96Wells
Marca : Aviva Systems Biology
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LPA ELISA Kit (Human) : 96 Wells (OKEH00633)
| Datasheets/Manuals | Printable datasheet for LPA ELISA Kit (Human) : 96 Wells (OKEH00633) |
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| COA Datasheet | Printable COA datasheet for OKEH00633 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology LPA ELISA Kit (Human) (OKEH00633) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LPA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LPA captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 3.9-250ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.2% (n = 20) Mean Inter-assay CV%: < 8.3% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 1.2 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Apolipoprotein(a) | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | LPA |
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| Gene Full Name | lipoprotein(a) |
| Alias Symbols | LP, AK38, APOA |
| NCBI Gene Id | 4018 |
| Protein Name | Apolipoprotein(a) |
| Description of Target | Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330. |
| Uniprot ID | P08519 |
| Protein Accession # | NP_005568.2 |
| Nucleotide Accession # | NM_005577.2 |
| Protein Size (# AA) | 4548 |
| Molecular Weight | 501 kDa |
