Levine EMB Agar
Catalog Number: B2017272 (500 g)
Levine EMB Agar is a selective and differential medium for isolating and differentiating Gram-negative enteric bacteria, particularly coliforms like E. coli, in water and food samples. It contains eosin Y, methylene blue, lactose, peptone, and agar. Lactose fermenters produce dark colonies with a metallic green sheen, while non-fermenters produce colorless or light pink colonies. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
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Product Description
Levine EMB Agar
Catalog number: B2017272
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 500 g
Molecular Weight or Concentration: N/A
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: RT
Keywords: Eosin methylene Blue Lactose Agar according to Levine, Levine’s Eosin Methylene Blue Agar
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Fredericq P. Eosin Methyl-Green Sulfite Agar: A Modification of Levine’s E.M.B. Agar J Bacteriol. 1947 Nov;54(5):662-3.
2: FREDERICQ P. Eosin methyl-green sulfite agar; a modification of Levine’s E.M.B. agar J Bacteriol. 1947 Nov;54(5):662.
3: Smith JL, Dell BJ. Capability of Selective Media to Detect Heat-Injured Shigella flexneri J Food Prot. 1990 Feb;53(2):141-144.
4: Resende MM, Rocha CA, Corrêa NF, Veiga RR, Passos SJ, Novo NF, Juliano Y, Damasceno CA. Tap water versus sterile saline solution in the colonisation of skin wounds Int Wound J. 2016 Aug;13(4):526-30.
5: Hinton A Jr, Cason JA. Bacterial flora of processed broiler chicken skin after successive washings in mixtures of potassium hydroxide and lauric acid J Food Prot. 2008 Aug;71(8):1707-13.
6: Feldsine P, Lienau AH, Shah K, Immermann A, Soliven K, Kaur M, Kerr DE, Jucker M, Hammack T, Brodsky M, Agin J. Comparison of Assurance GDS(®) MPX ID for Top STEC with Reference Culture Methods for the Detection of E. coli Top 6 STEC; Direct Confirmation of Top 6 STEC from Isolation Plates and Determination of Equivalence of PickPen(®) and FSIS OctoMACS™ Concentration Protocols J AOAC Int. 2016 Mar-Apr;99(2):428-43.
7: Fahim KM, Ismael E, Khalefa HS, Farag HS, Hamza DA. Isolation and characterization of E. coli strains causing intramammary infections from dairy animals and wild birds Int J Vet Sci Med. 2019 Dec 3;7(1):61-70.
8: Okrend AJG, Rose BE, Bennett B. A Screening Method for the Isolation of Escherichia coli O157:H7 from Ground Beef J Food Prot. 1990 Mar;53(3):249-252.
9: Huang SW, Chang CH, Tai TF, Chang TC. Comparison of the beta-glucuronidase assay and the conventional method for identification of Escherichia coli on eosin-methylene blue agar J Food Prot. 1997 Jan;60(1):6-9.
10: Stiles ME, Ng LK. Estimation of Escherichia coli in raw ground beef Appl Environ Microbiol. 1980 Aug;40(2):346-51.
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Additional Information
| Weight | 35 oz |
|---|---|
| Dimensions | 8 × 8 × 8 in |