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> L-Methionine [63-68-3]

L-Methionine [63-68-3]

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Referência HY-N0326-1mL

Tamanho : 10mM/1mL

Marca : MedChemExpress

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Description

L-Methionine is an L-isomer of orally active Methionine, an essential amino acid. Methionine is a strong liver antidote that acts as a liver protector. L-Methionine can inhibit cell proliferation and induce cell apoptosis. L-Methionine has antitumor and antioxidant activity[1][2][3].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

L-Methionine (5 mg/mL, 7 or 3 days) can inhibit the proliferation of BXPC-3 (mutant p53) and HPAC (wild-type p53) pancreatic cancer cells, interfere with cell cycle, and induce apoptosis of BXPC-3 cells[1].
L-Methionine (0.3-10 mM, 24 h) reduces the formation of free radicals in endothelial cells by inducing heme oxidase-1 and ferritin[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Methionine Related Antibodies

Cell Proliferation Assay[1]

Cell Line: BXPC-3, HPAC
Concentration: 5 mg/mL
Incubation Time: 7 or 3 days
Result: Reduced growth of BXPC-3 cells by 31 and 32%, respectively.
Reduced growth of HPAC cells by 35% and 18%, respectively.
Reduced S to G2 transition in BXPC-3 cells and intervened in both G1–S and S–G2 transitions in HPAC cells.
Increased late cell apoptosis at a percentage of 76% in BXPC-3 cells.

Western Blot Analysis[2]

Cell Line: ECV304
Concentration: 0.3, 1, 3, 5, 10 mM
Incubation Time: 24 h
Result: Increased the level of HO-1 protein up to 9.5-fold and the HO-1 activity.
In Vivo

L-Methionine (215, 268.8, 322.5, 430 mg/kg, orally, for 14 consecutive days) induces endogenous antioxidant activity by activating the Nrf2-ARE pathway and inhibits ROS-induced oxidative stress in growing rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (body weight 70-80 g)[3]
Dosage: 215,268.8, 322.5, 430 mg/kg
Administration: p.o.
Result: Reduced the hepatic ROS levels and the hepatic MDA contents.
Increased the hepatic T-AOC, CAT and T-SOD.
Increased the hepatic activity of GCL, GR, GST and GPx.
Masse moléculaire

149.21

Formule

C5H11NO2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCSC)C(O)=O
Structure Classification
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

H2O : 10 mg/mL (67.02 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7020 mL 33.5098 mL 67.0196 mL
5 mM 1.3404 mL 6.7020 mL 13.4039 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 12.5 mg/mL (83.77 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
Références

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