L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples)
Referência E-BC-K043-S-100
Tamanho : 100Assays
Marca : Elabscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples) (E-BC-K043-S)
For research use only.
| Detection Principle | Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm. |
| Sample Type | Whole blood |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (530 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.14 mmol/L |
| Detection Range | 0.14-7.0 mmol/L |
| Precision | inter-assay CV: 2.0% | intra-assay CV: 1.6% |
| Sample Volume | 0.05 mL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rabbit whole blood | 1-2 |
| Mouse whole blood | 1-2 |
| Rat whole blood | 1-2 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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