OARA05457 | IgM Antibody Quimigen Portugal

Rabbit Anti-Human IgM (Fc5u) Polyclonal Antibody (OARA05457)

Datasheets/Manuals	Printable datasheet for Rabbit Anti-Human IgM (Fc5u) Polyclonal Antibody (OARA05457)

Product Info
Predicted Species Reactivity	Human
Product Format	Liquid. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality	Polyclonal
Isotype	IgM
Conjugation	Unconjugated
Application	ELISA\|IHC\|WB
Reconstitution and Storage	-20°C\|2°C to 8°C
Immunogen	Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in rabbit.
Purification	Human IgM Fc5u Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Concentration	2.07 mg/ml
Specificity	IgM Fc5u. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA.
Application Info	ELISA: 1:20000~50000 IHC: 1:500~3000 WB: 1:500~3000 All assays should be optimized by the user. Human IgM Fc5u antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Intended Use	For research use only.

Description of Target	Anti-Human IgM Fc5u antibody generated in rabbit specifically detects Fc5u portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5u antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.