OKEH07750 | IDH1 ELISA Kit (Pig) Quimigen Portugal

Technical Documents

Datasheets/Manuals	Printable datasheet for IDH1 ELISA Kit (Pig) (OKEH07750)

Product Info

Predicted Species Reactivity

Porcine|Sus scrofa

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Linearity

Sample	1:2	1:4	1:8	1:16
serum(n=5)	114-124% (n = 20)	110-118% (n = 20)	88-100% (n = 20)	101-111% (n = 20)
EDTA plasma(n=5)	91-104% (n = 20)	109-118% (n = 20)	106-118% (n = 20)	96-105% (n = 20)
heparin plasma(n=5)	91-100% (n = 20)	90-99% (n = 20)	101-111% (n = 20)	89-101% (n = 20)

ELISA Kit Principle

Aviva Systems Biology IDH1 ELISA Kit (Pig) (OKEH07750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IDH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IDH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IDH1 captured in well.

ELISA Kit Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)

ELISA Kit Reproducibility

Mean Intra-assay CV%: <=4.7% (n = 20)
Mean Inter-assay CV%: <=5.8% (n = 20)

ELISA Kit Component

Component	Amount
IDH1 Microplate	96 Wells (12 x 8 Well strips)
IDH1 Lyophilized Standard	2
100X Biotinylated IDH1 Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Sample Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

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Unigene: Ssc.3518

Reconstitution and Storage

Store as indicated in product manual.

Specificity

Natural and recombinant Pig Isocitrate dehydrogenase [NADP] cytoplasmic

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	IDH1
Alias Symbols	Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase.
Protein Name	Isocitrate dehydrogenase [NADP] cytoplasmic
Description of Target	Isocitrate dehydrogenase [NADP] cytoplasmic
Uniprot ID	P20304

Protocols & Data

IDH1 ELISA Kit (Pig)

Referência OKEH07750

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Marca : Aviva Systems Biology

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IDH1 ELISA Kit (Pig) (OKEH07750)