Anti-Hexokinase (RABBIT) Antibody Peroxidase Conjugated, 100 ug
Size:
100 ug
Conjugation:
HRP
Western blot of rabbit anti-Hexokinase antibody peroxidase conjugated. Lane 1: Hexokinase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Hexokinase antibody peroxidase conjugated at 1/1,000 overnight at 4°C. Secondary antibody: n/a, blocking buffer for 30 min at RT. Predicted/Observed size: 54 kDa, 54 kDa for Hexokinase.
View other "HXK2 or CAA96973.1" antibodies
cDNA Clones
Lentiviral
AAV
CRISPR
RNAi
Proteins
Antibodies
Specifications
Product Data
Application
ELISA, WB
Recommended Dilution
WB: 1:500 - 1:1,000 ELISA: 1:1,000 - 1:2,500
Reactivity
Hexokinase (Yeast)
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Hexokinase Yeast
Specificity
Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase Yeast. Cross reactivity against Hexokinase from other tissues and species is expected based on sequence homology.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Reconstitution Method
Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration
1.0 mg/mL - lot specific
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation
HRP
Storage
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability
Expiration date is one (1) year from date of receipt.
HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.
Anti-HEXOKINASE Peroxidase Conjugated Antibody has been tested by ELISA and western blot and can be used in applications such as Immunohistochemistry (IHC), and Immunofluorescence (IF). Specific conditions should be optimized by researcher.
AI Summary: In this study, OriGene’s Tom20 cDNA (Cat# SC313192) and Tom20 antibody (Cat# TA309494) were used to investigate the role of Tom20 in mediating mRNA localization to mitochondria, enabling the authors to assess Tom20 function and protein levels in their experimental models.
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