Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Human TREM-2 ELISA Kit
Human TREM-2 ELISA Kit
RayBio® Human TREM-2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 28 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 28 pg/ml - 7,000 pg/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, Activin RIB, ADAM23, Artemin, Cardiotrophin-1, Cathepsin V, FABP1, FGF-20, GDF-8, HAI-1, IL-27 R alpha, Insulin R, Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR, Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2, p53, PDECGF, PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1, SREC-I, SREC-II, TLR1, TLR3, TPP1, TrkC, TROY, Uromodulin, XIAP. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q9NZC2 |
| Gene Id | 54209 |
| Gene Symbols | TREM2 |
| Protein Name / Synonyms | Triggering receptor expressed on myeloid cells 2 (TREM-2) (Triggering receptor expressed on monocytes 2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human TREM2 qIPCR ELISA Kit
As low as $749.00
RayPlex® Human TREM2 Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Customize This Human TREM-2 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified TREM-2 (TREM2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Decoding Parkinson's Disease: The interplay of cell death pathways, oxidative stress, and therapeutic innovations
- Year:
- 2025
- Journal Name:
- Redox Biology
- PubMed ID:
- 40712453
Macrophage TREM2 is a triglyceride-rich lipoprotein receptor that undergoes shedding in prediabetic hepatic steatosis
- Species:
- Human
- Sample Type:
- Plasma (fasting plasma)
- Year:
- 2026
- Journal Name:
- JCI Insight
- PubMed ID:
- 42658581
PPAR-γ activation by pioglitazone inhibits microglia-mediated neuroinflammation and alleviates disease in neuromyelitis optica spectrum disorders
- Year:
- 2025
- Journal Name:
- Acta Neuropathologica Communications
- PubMed ID:
- 41444620
Soluble TREM2 levels associate with conversion from mild cognitive impairment to Alzheimer’s disease
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Journal of Clinical Investigation
Association between sTREM2, an immune biomarker of microglial activation, and frontal lobe function in community-dwelling older adults: a cross-sectional study
- Species:
- Human
- Sample Type:
- Serum (community-dwelling older adults)
- Year:
- 2024
- Journal Name:
- Journal of rural medicine : JRM
- PubMed ID:
- 38975040
sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture media of M0, M1, M2 macrophages)
- Year:
- 2024
- Journal Name:
- Biology-Basel
- PubMed ID:
- 38392305
Serum soluble triggering receptor expressed on myeloid cells-2 was not altered by rTMS in patients with treatment-resistant depression
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Neuropsychopharmacology Reports
Soluble TREM2 and Alzheimer-related biomarker trajectories in the blood of patients with diabetes based on their cognitive status
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Diabetes Research and Clinical Practice
Association Between sTREM2, an Immune Biomarker of Microglial Activation, and Aging-Related Brain Volume Changes in Community-Dwelling Older Adults: A 7-Year Follow-Up Study
- Species:
- Human
- Sample Type:
- Serum (community-dwelling older adults)
- Year:
- 2021
- Journal Name:
- Frontiers in Aging Neuroscience
- PubMed ID:
- 33981209
CSF biomarker profiles in CNS infection associated with HSV and VZV mimic pattern in Alzheimer’s disease
- Species:
- Human
- Sample Type:
- Cerebrospinal Fluid (HSV and VZV CNS infection)
- Year:
- 2020
- Journal Name:
- Research Square