Background: The Slc26 family is a conserved family of anion transporters. In the human, their physiological relevance was highlighted with the discovery of pathogenic mutations in several Slc26 transporters that lead to distinctive clinical disorders (Pendred syndrome, deafness, diastrophic dysplasia, congenital chloride diarrhoea) that are related to the specific distribution of these genes. We previously identified TAT1 as a new family member (Slc26A8), very specifically expressed in male germ cells in both the human and the mouse.

Principle of the Assay: Human Testis Anion Transporter 1 (SLC26A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC26A8 in samples. An antibody specific for SLC26A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC26A8 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SLC26A8 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SLC26A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of TAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9714275 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Testis Anion Transporter 1 (SLC26A8) (TAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TAT1. The ELISA analytical biochemical technique of the MBS9714275 kit is based on TAT1 antibody-TAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).