RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human MCP-1 ELISA Kit
Human MCP-1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 500 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P13500 |
| Gene Id | 6347 |
| Gene Symbols | CCL2, SCYA2, MCP1 |
| Protein Name / Synonyms | C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human MCP-1 (CCL2) qIPCR ELISA Kit
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Target Background
The monocyte chemoattractant protein-1 (MCP-1/CCL2) is a potent chemotactic factor for monocytes. MCP-1 is made of 76 amino acids and is 13 kDa in size. It is produced by a variety of different cell types such as, endothelial, fibroblasts, epithelial, smooth muscle, astrocytic, monocytic and microglial cells. MCP-1/CCL2 mediates its effects through the receptor, CCR2. Genetic variants of MCP-1/CCL2 affect serum levels and the incidence of myocardial infarction. Both MCP-1/CCL2 and its receptor have been associated with diseases such as thrombus formation, tuberculosis, cancer, HIV and multiple sclerosis.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human MCP-1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MCP-1 (CCL2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Transglutaminase 2 function in glioblastoma tumor efferocytosis
- Species:
- Human
- Sample Type:
- Other / Unspecified (secreted CCL2 produced by glioblastoma cells)
- Year:
- 2025
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 40610434
Urinary Cytokeratin 20 as a Biomarker for AKI-CKD Transition among Patients with Acute Decompensated Heart Failure and Acute Kidney Injury
- Year:
- 2025
- Journal Name:
- Journal of the American Society of Nephrology
- PubMed ID:
- 39466275
Stacked human aortic endothelial cells induce atherosclerotic fatty streaks and release proinflammatory cytokines and chemokines
- Year:
- 2026
- Journal Name:
- Mechanobiol Med
- PubMed ID:
- 42088731
Effects of Regular Exercise on the Biochemical, Oxidative, and Inflammatory Profiles and Quality of Life in Older Spaniards with Metabolic Syndrome
- Species:
- Human
- Sample Type:
- Plasma (older adults with metabolic syndrome)
- Year:
- 2024
- Journal Name:
- Antioxidants
- PubMed ID:
- 38671898
The Anticancer Effect of Kaempferol Through Downregulation of CDKs and PD-L1 in Triple-Negative Breast Cancer Cells
- Species:
- Human
- Sample Type:
- Other / Unspecified (protein release from MDA-MB-231, MDA-MB-468 cells)
- Year:
- 2025
- Journal Name:
- Cancers
- PubMed ID:
- 41463161
Elevated IL-6 Expression in Autologous Adipose-Derived Stem Cells Regulates RANKL Mediated Inflammation in Osteoarthritis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants of ASCs)
- Year:
- 2024
- Journal Name:
- Cells
- PubMed ID:
- 39768138
Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (SSc patient PBMCs treated with IL-40)
- Year:
- 2025
- Journal Name:
- Arthritis Research & Therapy
- PubMed ID:
- 40457473
Oxidative Stress and Inflammatory Biomarkers Are Related to High Intake of Ultra-Processed Food in Old Adults with Metabolic Syndrome
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Antioxidants
Long-Term Impact of Nutritional Intervention with Increased Polyphenol Intake and Physical Activity Promotion on Oxidative and Inflammatory Profiles in Patients with Metabolic Syndrome
- Species:
- Human
- Sample Type:
- Plasma (metabolic syndrome patients)
- Year:
- 2024
- Journal Name:
- Nutrients
- PubMed ID:
- 38999869
The prognostic significance of CCL2 in oral squamous cell carcinoma: insights from tissue expression and perioperative serum profiling
- Species:
- Human
- Sample Type:
- Serum (peripheral blood serum samples)
- Year:
- 2026
- Journal Name:
- Frontiers in Oncology
- PubMed ID:
- 42038362
