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Human Lipocalin-2 ELISA Kit
Human Lipocalin-2 ELISA Kit
RayBio® Human Lipocalin-2 (NGAL) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 4 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 4 pg/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 200 fold |
| Accession Number | P80188 |
| Gene Id | 3934 |
| Gene Symbols | LCN2, HNL, NGAL |
| Protein Name / Synonyms | Neutrophil gelatinase-associated lipocalin (NGAL) (25 kDa alpha-2-microglobulin-related subunit of MMP-9) (Lipocalin-2) (Oncogene 24p3) (Siderocalin LCN2) (p25) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
Recombinant Human NGAL Protein
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NexaTag® Human Lipocalin-2 qIPCR ELISA Kit
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RayPlex® Human Lipocalin-2 Bead Assay
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Lipocalin-2 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Lipocalin-2 (NGAL / LCN2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Procalcitonin levels in septic and nonseptic subjects with AKI and ESKD prior to and during continuous kidney replacement therapy (CKRT)
- Species:
- Human
- Sample Type:
- Plasma (AKI/ESKD patients on CKRT)
- Year:
- 2025
- Journal Name:
- Critical Care
- PubMed ID:
- 40307866
The utility of early gestational OGTT and biomarkers for the development of gestational diabetes mellitus: an international prospective multicentre cohort study
- Species:
- Human
- Sample Type:
- Serum (maternal serum)
- Year:
- 2025
- Journal Name:
- Diabetologia
- PubMed ID:
- 40817933
Potential Utility of Combined Urine Lipocalin‑2 and Copper Test in Diagnosing Acute Pyelonephritis or Cystitis
- Species:
- Human
- Sample Type:
- Urine (pyelonephritis/cystitis patients)
- Year:
- 2025
- Journal Name:
- ACS Omega
- PubMed ID:
- 41048711
Quantification of intra-amniotic inflammation in late preterm prelabour rupture of membranes
- Species:
- Human
- Sample Type:
- Other Body Fluid (amniotic fluid)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 40295647
Prognostic value, biological role, and mechanisms of LCN2 in childhood acute lymphoblastic leukemia
- Species:
- Human
- Sample Type:
- Cerebrospinal Fluid (childhood ALL); Plasma; Cell Culture Supernatant
- Year:
- 2025
- Journal Name:
- American Journal of Cancer Research
- PubMed ID:
- 40371147
Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (smokers vs non-smokers, breast cancer); Serum; Urine
- Year:
- 2021
- Journal Name:
- Nature Communications
- PubMed ID:
- 33473115
Neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, and periostin: Novel urinary biomarkers in diabetic nephropathy
- Species:
- Human
- Sample Type:
- Urine (diabetic patients and healthy comparators)
- Year:
- 2024
- Journal Name:
- World journal of nephrology
- PubMed ID:
- 39723350
Effect of sodium bicarbonate on performance and markers of kidney and gastrointestinal injury during repeated sprints in the heat
- Species:
- Human
- Sample Type:
- Urine (repeated sprints in heat, NaHCO3 vs placebo)
- Year:
- 2026
- Journal Name:
- European Journal of Applied Physiology
- PubMed ID:
- 42133056
Gastrointestinal permeability and kidney injury risk during hyperthermia in young and older adults
- Species:
- Human
- Sample Type:
- Urine (young and older adults, passive hyperthermia)
- Year:
- 2025
- Journal Name:
- Experimental Physiology
- PubMed ID:
- 39417775
Inflammatory milieu in contrast-induced nephropathy: a prospective single-center study
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- International journal of nephrology and renovascular disease
