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> Human Kappa Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

Human Kappa Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

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Referência R1401HRP

Tamanho : 1mg

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Human Kappa Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

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SKU
R1401HRP
Goat F(ab')2 Anti-Human κ (kappa chain) Antibody Peroxidase Conjugated, 1 mg
 
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000
Reactivity Human
Antibody Host Goat
Clonality Polyclonal
Immunogen Human kappa light chain
Specificity F(ab')2 Anti-Human (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Buffer 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Thimerosal
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 1.0 mg/mL - lot specific
Purification Immunoaffinity chromatography.
Conjugation HRP
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note F(ab')2 anti-Human (kappa chain) Peroxidase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of human kappa light chain in a standard capture ELISA using ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
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FAQs
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SDS
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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