- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human IMA antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IMA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
Human Ischemia Modified Albumin,IMA ELISA Kit
Referência CSB-E09594h-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Ischemia Modified Albumin,IMA
Abbreviation
IMA
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 IU/mL-200 IU/mL
Sensitivity
0.78 IU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human IMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
Recovery
| The recovery of human IMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 91-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human IMA ELISA Kit was designed for the quantitative measurement of Human IMA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 IU/mL-200 IU/mL and the sensitivity is 0.78 IU/mL .
Citations
- D Kurt, P Yıldız,Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi,2026
- P Resano-Barrio, E Gracia-Lavedan, E Solano-Pérez,Sleep Medicine,2025
- K Tamme,BMJ open,2024
- H Memmedov,The Pan African medical journal,2023
- P Resano-Barrio,International journal of molecular sciences,2023
- SS Ahn,PloS one,2022
- J Chen,BMC Womens Health,2020
- Zebrowska A, et al,Journal of Clinical Medicine,2019
- Pipikos T.et al,J Nucl Cardiol,2018
- Ischemic modified a
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