Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]

Specifications

Product Data
Clone Name	NI 330 (HP 6050)
Application	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. General Recommended Dilutions: Histochemical Use: 1/10-1/50. ELISA: from 1/200 upwards. Western blot: from 1/400 upwards.
Reactivity	Human
Antibody Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified monoclonal IgG3 isolated from Human serum.
Specificity	The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab’)2 part of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer	PBS, pH 7.2 without preservatives and foreign proteins Label: TRITC State: Lyophilized purified IgG fraction Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm / 573 nm Molar radio: Fluorochrome/IgG ~2.0
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water
Concentration	0.4 mg/ml
Conjugation	TRITC
Storage	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.
Shipping	Ambient

Product Manuals

Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit

FAQs

Antibody and Western Standard

SDS

SDS for AM20255TC-N

Antibody Resources

Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads