Quimigen Portugal > Human IgG1 Kappa Antibody : AP
Human IgG1 Kappa Antibody : AP
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Human IgG1 Kappa Antibody : AP (OASB00147)
| Datasheets/Manuals | Printable datasheet for Human IgG1 Kappa Antibody : AP (OASB00147) |
|---|
| Clonality | Purified from myeloma serum |
|---|---|
| Isotype | IgG1 |
| Host | Human |
| Conjugation | AP |
| Reconstitution and Storage | Store at 2-8C |
| Purification | Affinity chromatography on protein G covalently linked to agarose |
| Concentration | Lot specific |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA and immunoelectrophoresis toconform with characteristics of a standard reference reagent. In accordance with current GoodManufacturing and Good Laboratory Practices (cGMP/cGLP), any protein of human blood origin should behandled pursuant to your organization's documented safety procedures and as if it is capable of transmittinginfection. This product has NOT been tested for viral, bacterial, or other infectious agents such as, but notlimited to, HIV, HbsAg, HCV. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoicacid under acidic conditions. Dilute azide- containing compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Optimal dilutions for use of this product vary according to specific applications and should be determined by the individual investigator. |
| Purity | >95% as judged by SDS- polyacrylamide gel electrophoresis. |
| Application Info | Enzyme-Linked-Immunosorbent-Assay (ELISA), SDS-PAGE and immunoblotting |
| Storage | - The purified (UNLB) IgG is provided at a concentration of 0.5 mg/mL in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. The human IgGsolution has been centrifuged and passed through a 0.22 u filter, and is free of any visible precipitate The product should be stored at 2- 8 C and is stable for the period shown on the label if stored asdirected - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C. |
| Gene Full Name | Ig kappa chain C region |
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