Human IgG (H+L) Antibody : FITC
Referência OASB01995
Tamanho : 0.5mg
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
Rabbit Anti-Human IgG(H+L) Antibody - FITC Conjugated (OASB01995)
| Datasheets/Manuals | Printable datasheet for Rabbit Anti-Human IgG(H+L) Antibody - FITC Conjugated (OASB01995) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | FITC |
| Application | FLISA, ICC, WB |
| Additional Information | Description: Pepsin digest of Rabbit Anti-Human IgG(H+L) - Pooled antisera from rabbits hyperimmunized with human IgG; purified by affinity chromatography on human IgG covalently linked to agarose; reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Human IgG |
| Purification | Affinity chromatography on human IgG covalently coupled to agarose. Gelfiltration chromatography of pepsin digested antibody |
| Concentration | 0.5 mg/mL |
| Specificity | IgG(H+L) |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA and/or Fluorescent ELISA forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence ELISA: FITC conjugate AP conjugate HRP conjugate <= 1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 |
| Application Info | Immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | NA |
| Gene Full Name | Ig gamma-1 chain C region |
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