Human IgG (Fc) Antibody : AP

Referência OASB00875

Tamanho : 1.0mL

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Human IgG (Fc) Antibody : AP (OASB00875)
Product Info
Tested Species ReactivityHuman
Predicted Species ReactivityHuman
ClonalityMonoclonal
CloneJDC-10
IsotypeIgG1
HostMouse
ConjugationAP
ApplicationIHC-P, WB
Reconstitution and StorageStore at 2-8C
ImmunogenPurified human IgG1 myeloma protein
ConcentrationLot specific
SpecificityIgG Fc
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
ELISA:
Purified antibody
BIOT conjugate
R-PE conjugate
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.2 ug/106 cells
1:1,000-1:4,000
1:2,000-1:8,000
1:5,000-1:10,000
Application InfoIndirect immunofluorescent staining of IgG+ human B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry (frozen sections), Immunocytochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Each reagent is stable for the period shown on the bottle label if stored as directed.
Gene Full NameIg gamma-1 chain C region