Specifications

Product Data
Application	ELISA, ID, IF, IHC, IP
Recommended Dilution	Can be used for direct Immunofluorescence staining of cytoplasmic IgE of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/30.
Reactivity	Human
Antibody Host	Goat
Isotype	IgG
Clonality	Polyclonal
Immunogen	Purified monoclonal IgE isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
Buffer	PBS, pH 7.2 without preservatives and foreign proteins Label: TRITC State: Lyophilized purified IgG fraction Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm / 573 nm Molar radio: TRITC/IgG: ~2.0
Reconstitution Method	Restore by adding 1,0 ml sterile distilled water.
Concentration	10,0 mg/ml
Purification	Immunoaffinity Chromatography
Conjugation	TRITC
Storage	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shipping	Ambient
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies crossreacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Product Manuals

Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit

FAQs

Antibody and Western Standard

SDS

SDS for AP21480TC-N

Antibody Resources

Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads