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Human HIF-1 alpha ELISA Kit
Human HIF-1 alpha ELISA Kit
RayBio® Human HIF-1 alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Mouse |
| Sensitivity | 61 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 61 pg/ml - 15 ng/ml |
| Specificity | This ELISA antibody pair detects human and mouse HIF-1apha. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q16665 |
| Gene Id | 3091 |
| Gene Symbols | HIF1A, BHLHE78, MOP1, PASD8 |
| Protein Name / Synonyms | Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha) (ARNT-interacting protein) (Basic-helix-loop-helix-PAS protein MOP1) (Class E basic helix-loop-helix protein 78) (bHLHe78) (Member of PAS protein 1) (PAS domain-containing protein 8) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation, HIF-1 alpha Signaling, Autophagy, Hypoxia Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
Recombinant Human HIF-1-alpha Protein
As low as $94.00
NexaTag® Human HIF-1 alpha qIPCR ELISA Kit
As low as $899.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human HIF-1 alpha ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified HIF-1 alpha (HIF1A), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
The HCG11/QKI5 Axis Regulates Endothelial Angiogenic Adaptation During Chronic Cerebral Hypoperfusion
- Species:
- Human
- Sample Type:
- Plasma (chronic cerebral hypoperfusion patients)
- Year:
- 2026
- Journal Name:
- CNS Neuroscience & Therapeutics
- PubMed ID:
- 42484275
Altered Monocyte and Lymphocyte Phenotypes Associated with Pathogenesis and Clinical Efficacy of Progestogen Therapy for Peritoneal Endometriosis in Adolescents
- Species:
- Human
- Year:
- 2024
- Journal Name:
- Cells
- PubMed ID:
- 39056769
Imaging of Angiogenesis in White Matter Hyperintensities
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of the American Heart Association
Helpful to Live Healthier? Intermittent Hypoxic/Ischemic Training Benefits Vascular Homeostasis and Lipid Metabolism with Activating SIRT1 Pathways in Overweight/Obese Individuals
- Species:
- Human
- Sample Type:
- Serum (overweight/obese individuals)
- Year:
- 2024
- Journal Name:
- Obesity facts
- PubMed ID:
- 38185107
Exploring Visceral and Subcutaneous Adipose Tissue Secretomes in Human Obesity: Implications for Metabolic Disease
- Species:
- Human
- Sample Type:
- adipose tissue
- Year:
- 2022
- Journal Name:
- Endocrinology
Guava Leaf Extract Suppresses Fructose Mediated Non-Alcoholic Fatty Liver Disease in Growing Rats
- Species:
- Human
- Sample Type:
- HepG2 cell
- Year:
- 2022
- Journal Name:
- Diabetes Metabolic Syndrome and Obesity-Targets and Therapy
Altered Amyloid-β and Tau Proteins in Neural-Derived Plasma Exosomes in Patients with Type 2 Diabetes and Orthostatic Hypotension
- Species:
- Human
- Sample Type:
- Serum (T2D patients with orthostatic hypotension)
- Year:
- 2020
- Journal Name:
- Research Square
Thyroid-related hormones as potential markers of hypoxia/ischemia
- Species:
- Human
- Sample Type:
- Other / Unspecified (UD-PTC cell perfusion culture)
- Year:
- 2020
- Journal Name:
- Human Cell
- PubMed ID:
- 32146707
Aegle marmelos differentially affects hepatic markers of glycolysis, insulin signalling pathway, hypoxia, and inflammation in HepG2 cells grown in fructose versus glucose-rich environment.
- Species:
- human
- Sample Type:
- Cell Lysate (human heptaocellular)
- Year:
- 2018
- Journal Name:
- Molecular and Cellular Biochemistry
- PubMed ID:
- 28766170
Genetic and immune changes in Tibetan high-altitude populations contribute to biological adaptation to hypoxia
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Environmental health and preventive medicine
