- A 96-well Assay plate --The 96-well plate has been pre-coated with HEV antigen
- Negative Control (1 x 0.4ml) --Eliminate false positive
- Positive Control (1 x 0.4ml) --Used to evaluate the validity, stability, and comparability of experimental results.
- HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HEV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (1x 12ml) --Dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
Human Hepatitis E virus antibody(IgG)ELISA Kit
Referência CSB-E04811h-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Hepatitis E virus antibody(IgG)
Abbreviation
HEV Ab (IgG)
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
Request Information
Sensitivity
Request Information
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Microbiology
Assay Principle
qualitative
Measurement
Indirect
Precision
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||||
Typical Data
Test parameter | specification | test result | |||||
Positive control | >0.30 | 0.799 | |||||
Negative control | <0.08 | 0.046 | |||||
Positive rate | 10，Positive | 100% | |||||
Negative rate | 10，Negative | 100% | |||||
Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human Hepatitis E virus antibody (IgG) ELISA Kit is designed to qualitatively detect the HEV IgG antibody in the serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HEV antigen. Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human HEV IgG antibody bound in the initial step. The color development is stopped and the intensity of the color...
Citations
- MF Menajovsky, J Espunyes, G Ulloa, S Montero,Microorganisms,2024
- Neziha Yilmaz.et al,/,2016
- Klonizakis P.et al,Vox Sang,2017
Customer Reviews and Q&A
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■ Q&A
Q:
1.#CSB-E04811h,Human Hepatitis E virus antibody(IgG)ELISA Kit
2.#CSB-E04812hHuman Hepatitis E virus antibody(IgM)ELISA Kit what is the difference between 1 and 2? we need for human use. do these have ce?
A:
Thanks for your inquiry. CSB-E04811h is used to detect IgG and CSB-E04812h is used to detect IgM. I will send you the kit manuals by email.
As our elisa kits are only for research purpose,not for diagnosis,we did not go to get CE certificate.
As our elisa kits are only for research purpose,not for diagnosis,we did not go to get CE certificate.