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Human GM-CSF ELISA Kit
Human GM-CSF ELISA Kit
RayBio® Human GM-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 500 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P04141 |
| Gene Id | 1437 |
| Gene Symbols | CSF2, GMCSF |
| Protein Name / Synonyms | Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF) (Molgramostin) (Sargramostim) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human GM-CSF qIPCR ELISA Kit
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RayPlex® Human GM-CSF Bead Assay
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human GM-CSF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified GM-CSF (CSF2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Mass drug administration of praziquantel lowers the susceptibility of school-aged children to Schistosoma mansoni in endemic areas
- Species:
- Human
- Sample Type:
- Plasma (school-aged children, endemic area)
- Year:
- 2026
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 41822487
Reduced plasma levels of GM-CSF is a common feature of Schistosoma mansoni-infected school-aged children
- Species:
- Human
- Sample Type:
- Plasma (school-aged children, S. mansoni-infected and controls)
- Year:
- 2025
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 40092989
RNA N6-Methyladenosine (m6A) Methyltransferase-like 3 Facilitates Tumorigenesis and Cisplatin Resistance of Arecoline-Exposed Oral Carcinoma
- Species:
- Human
- Sample Type:
- oral squamous cell carcinoma (OSCC) tissue
- Year:
- 2022
- Journal Name:
- Cells
Novel cytokine-antibody fusion protein, N-820, to enhance the functions of ex vivo expanded natural killer cells against Burkitt lymphoma
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatants of expanded NK cells, burkitt lymphoma co-cultures)
- Year:
- 2020
- Journal Name:
- Journal for Immunotherapy of Cancer
- PubMed ID:
- 33109629
Metal‐HisTag coordination for remote loading of very small quantities of biomacromolecules into PLGA microspheres
- Species:
- Human
- Sample Type:
- Other / Unspecified (protein released from PLGA microspheres)
- Year:
- 2021
- Journal Name:
- Bioengineering & Translational Medicine
- PubMed ID:
- 35600641
HIV induces airway basal progenitor cells to adopt an inflammatory phenotype
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatants from airway basal cell cultures)
- Year:
- 2021
- Journal Name:
- Scientific Reports
- PubMed ID:
- 33597552
Upregulated immuno-modulator PD-L1 in malignant peripheral nerve sheath tumors provides a potential biomarker and a therapeutic target
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- Cancer Immunology, Immunotherapy
FSTL1 promotes alveolar epithelial cell aging and worsens pulmonary fibrosis by affecting SENP1-mediated DeSUMOylation
- Species:
- Human
- Sample Type:
- Alveolar epithelial cell (AEC)
- Year:
- 2023
- Journal Name:
- Cell Biology International
A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages
- Species:
- Human
- Sample Type:
- Conditioned Media (Mammary Epithelial Cell lines)
- Year:
- 2014
- Journal Name:
- Nature Cell Biology
Relationships Between a Range of Inflammatory Biomarkers and Subjective Sleep Quality in Chronic Insomnia Patients: A Clinical Study
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Nature and science of sleep
