Human FCN1 (Ficolin-1) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	FCN1
Gene ID:	2219
Uniprot ID:	FCN1_HUMAN
Specificity:	This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes.
Function	Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.
Protein Name	Ficolin-1 Collagen/Fibrinogen Domain-Containing Protein 1 Ficolin-A Ficolin-Alpha M-Ficolin
Database Links	Reactome: R-HSA-166662 Reactome: R-HSA-166663 Reactome: R-HSA-2855086 Reactome: R-HSA-6798695
Cellular Localisation	Secreted Cell Membrane Peripheral Membrane Protein Extracellular Side Found On The Monocyte And Granulocyte Surface
Alternative CLIA Names	Ficolin-1 CLIA kit Collagen/Fibrinogen Domain-Containing Protein 1 CLIA kit Ficolin-A CLIA kit Ficolin-Alpha CLIA kit M-Ficolin CLIA kit FCN1 CLIA kit FCNM CLIA kit
Specificity	This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-97	92
EDTA plasma(n=8)	98-111	104
Cell culture media(n=8)	85-99	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	94.89	248.25	947.31	94.87	250.57	976.47
Standard deviation	10.68	29.47	104.01	8.07	27.79	104.29
CV (%)	11.26	11.87	10.98	8.51	11.09	10.68

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance