Human CD3 Antibody : LE/AF
Referência OASB00988
Tamanho : 0.5mg
Marca : Aviva Systems Biology
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Human CD3 Antibody : LE/AF (OASB00988)
| Datasheets/Manuals | Printable datasheet for Human CD3 Antibody : LE/AF (OASB00988) |
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| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | UCHT1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IHC-P, ICC, IP, WB, BL |
| Additional Information | Description: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa epsilon chain of the CD3/TCR complex. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Human peripheral blood lymphocytes from a patient with Sezary's syndrome and infant thymocytes |
| Concentration | 0.5 mg/mL |
| Specificity | CD3 |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin and Allophycocyanin-Cyanine 7 conjugates Spectral Red conjugate Cyanine 5 conjugate R-phycoerythrin-Cyanine 5.5 conjugate R-phycoerythrin-Cyanine 7 conjugate AF488 and AF647 <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections)6, Immunoprecipitation, In vitro activation7 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647)conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), R- phycoerythrin- Cyanine 5.5(R- PE- CY 5.5), and allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 100 tests in1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, OxfordUniversity Press, Oxford 2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 3. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd,eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford 4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFactsBook, 2nd Edition, CD3 Section, Academic Press, New York, p. 137 5. Beverly, P.C.L., and P.E. Callard. 1981. Eur. J. Immunol. 11:329 6. Baeten, D., Demetter, P., Cuvelier, C., Van den Bosch, F., Kruithof, E., Van Damme, N., Verbruggen, G., Mielants, H., Veys, E.M., and F. DeKeyser, eds. 2000. Ann Rheum Dis. 59:945- 953 7. Viglietta, V., Baecher- Allen, C., Weiner, H.L., and D. A. Hafler, eds. 2004. J. Exp. Med. 199(7):971- 979. |
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| Gene Symbol | CD3E |
| Gene Full Name | T-cell surface glycoprotein CD3 epsilon chain |
| Alias Symbols | T3E, TCRE, IMD18 |
| NCBI Gene Id | 916 |
| Protein Name | T-cell surface glycoprotein CD3 epsilon chain |
| Description of Target | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| Uniprot ID | P07766 |
| Protein Accession # | NP_000724.1 |
| Nucleotide Accession # | NM_000733.3 |
