This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Anti SARS-CoV-2 UK Variant IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti SARS-CoV-2 UK Variant IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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| 96T | Inquiry |
- Product Name
- Human Anti SARS-CoV-2 (COVID) Spike RBD Alpha B.1.1.7 Variant (UK Variant) IgM ELISA Kit
- Alias
- SARS-CoV-2 UK Variant IgM ELISA Kit
- Catalogue No.
- EH5031
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Indirect ELISA Sandwich
- Detection Wavelength
- OD450
- Reaction Duration
- 3 hours
- Range
- 0.781-50ng/ml
- Sensitivity
- 0.469ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with Anti SARS-CoV-2 UK Variant IgM , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
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- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
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- Standard Curve
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This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.05 0.052 0.051 0.781 0.125 0.129 0.127 1.563 0.235 0.241 0.238 3.125 0.396 0.408 0.402 6.25 0.632 0.65 0.641 12.5 0.871 0.897 0.884 25 1.607 1.653 1.63 50 2.162 2.224 2.193 Product Comparison