Human Angiotensin 1-7(Ang1-7)ELISA Kit
Referência CSB-E14242h-24T
Tamanho : 24T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Angiotensin 1-7(Ang1-7
Abbreviation
Ang1-7
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 85-109 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 92-114 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-110 | |||||
Recovery
| The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-110 | ||||
| EDTA plasma (n=4) | 99 | 90-108 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human Ang1-7 ELISA Kit was designed for the quantitative measurement of Human Ang1-7 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
Citations
- AML Quek, O Teng, JH Park, BGC Er, ECH Lim,Experimental and Molecular Pathology,2025
- Y Chen, Y Jin,Scientific Reports,2025
- S Tian, T Wu, Z Zhang, S Lv, X Ji,International Journal of Biological Sciences,2025
- E Duijvelaar,Critical care,2024
- F Chen,Journal of translational medicine,2023
- Zilun L,Cell Metabolism,2022
- Neza Alfazema, et al,Hypertension,2019
- Ming Zhang, et al,Biomed Research International,2019
- Xuemin G, et al,Experimental Physiology,2019
- Nie DM. et al,J Huazhong Univ Sci Technolog Med Sci,2015
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