HRP Conjugated Goat Anti-human IgA (alpha-chain specific) secondary antibody
Referência MBS176676-0,5mL
Tamanho : 0.5mL
Marca : MyBiosource
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Conjugated Goat Anti-Human IgA (HRP) Secondary Antibody – Goat Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176676Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for human IgA. More details
Product Overview
Online Datasheet in PDFProduct Name
HRP Conjugated Goat Anti-human IgA, Secondary Antibody
Full Product Name
HRP Conjugated Goat Anti-human IgA (alpha-chain specific) secondary antibody
Clonality
Polyclonal
Isotype
IgA
Host
Goat
Form/Format
Concentrated, Liquid
Concentration
0.5mg/ml (lot specific)
Specificity
This peroxidase conjugated antibody is specific for human IgA and shows no cross-reactivity with human IgGlIgM.
Preparation and Storage
At 4 degree C for one year.
Immunogen
Human IgA (whole molecule).
Contents
0.5 mg of peroxidase conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.
Labeling Method
Goat anti-humnn IgA is conjugated to ptOTOxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
Preparation of Diluent Buffer
Use 0.01 M TBS or 0.01 M PBS to dilute. See "Recommended Dilutions" below for details.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total Volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total Volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for HRP Conjugated Goat Anti-human IgA secondary antibody
Dot Blot (DB), Western Blot (WB), ELISA (EIA)
Application Notes for HRP Conjugated Goat Anti-human IgA secondary antibody
Dot blot (Enhanced chemiluminescent coloration): 1:2000-4000
WB (DAB coloration): 1:300-1000
WB (Enhanced chemiluminescent coloration): 1:2000-4000
ELISA (TMB coloration): 1 :20000-40000
Optimal working dilutions must be determined by end users.
WB (DAB coloration): 1:300-1000
WB (Enhanced chemiluminescent coloration): 1:2000-4000
ELISA (TMB coloration): 1 :20000-40000
Optimal working dilutions must be determined by end users.
Other Notes
Small volumes of HRP Conjugated Goat Anti-human IgA secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HRP Conjugated Goat Anti-human IgA purchase
MBS176676 is a secondary antibody targeting HRP Conjugated Goat Anti-human IgA. Also known as HRP Conjugated Goat Anti-human IgA (alpha-chain specific) secondary antibody. Host: Goat.
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