Horse insulin (INS) ELISA kit
Referência CSB-EL011742HO-5x96T
Tamanho : 5x96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
insulin
Associated names
INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
Abbreviation
Uniprot No.
Species
Equus caballus (Horse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 nIU/mL-100 nIU/mL
Sensitivity
0.39 nIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of horse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:2000 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 94-102 | |||||
Recovery
| The recovery of horse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-92 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Horse INS ELISA Kit was designed for the quantitative measurement of Horse INS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 nIU/mL-100 nIU/mL and the sensitivity is 0.39 nIU/mL.
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Target Background
Function
(From Uniprot)
(From Uniprot)
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location
Secreted.
Protein Families
Insulin family