HIVEP2 ELISA Kit (Human)
Referência OKEH03170
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
HIVEP2 ELISA Kit (Human) (OKEH03170)
| Datasheets/Manuals | Printable datasheet for HIVEP2 ELISA Kit (Human) (OKEH03170) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology HIVEP2 ELISA Kit (Human) (OKEH03170) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human HIVEP2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HIVEP2 and incubated. The HIVEP2 found in the sample or standards competes with the biotinylated HIVEP2 for limited binding sites on the immobilized anti-human HIVEP2 antibody. Excess unbound biotinylated HIVEP2 and sample or standard HIVEP2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HIVEP2 captured in the well and inversely proportional to the amount of HIVEP2 which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 6.7% (n = 20) Mean Inter-assay CV%: <= 8.0% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.156 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Transcription factor HIVEP2 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | HIVEP2 |
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| Gene Full Name | HIVEP zinc finger 2 |
| Alias Symbols | c-myc intron binding protein 1, HIV-EP2, human immunodeficiency virus type I enhancer binding protein 2, Human immunodeficiency virus type I enhancer-binding protein 2, MBP-2, MHC binding protein-2, MHC-binding protein 2, MIBP1, MRD43, Schnurri-2, SHN2, transcription factor HIVEP2, ZAS2, ZNF40B. |
| NCBI Gene Id | 3097 |
| Protein Name | Transcription factor HIVEP2 |
| Description of Target | This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation. |
| Uniprot ID | P31629 |
| Protein Accession # | NP_006725.3 |
| Nucleotide Accession # | NM_006734.3 |
| Protein Size (# AA) | 2446 |
| Molecular Weight | 269 kDa |