Specifications

Product Data
Expression Host	S. cerevisiae
Predicted MW	24 kDa
Concentration	lot specific
Purity	95% by SDS-PAGE
Presentation State	Purified
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: PBS, pH 7.2 without preservatives
Preparation	Liquid purified protein
Recommended Dilution	ELISA. Western Blot.
Protein Description	Recombinant Hepatitis B Surface Antigen (HBsAg) (ay) Recombinant HBsAg subtype ay. Genotype A. Does not contain a fusion partner.
Note	Centrifuge before opening to ensure complete recovery of vial contents.
Storage	Store the antigen at 2-8°C. DO NOT FREEZE!
Stability	Shelf life: one year from despatch.
Shipping	Blue Ice
Reference Data
Synonyms	HBV surface antigen, Hepatitis B Virus
Summary	Hepatitis B Virus (HBV) infection induces a disease state characterized by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
Protein Families	ELISA. Western Blot.

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SDS

SDS for AR31018PU-N

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein