Tamanho : 5mg
Telefone : +1 850 650 7790
Synonyms:
| Description | Heparan sulfate is a natural product, a linear polysaccharide. Heparan sulfate occurs as a proteoglycan (HSPG) that is tightly attached to cell surface or extracellular matrix proteins. |
| Targets & IC50 | MCF-10A cells (AR-induced mitogenesis):2 μg/mL, RBD:0.02 μg/mL |
| In vitro | METHODS: Breast cancer cells MDA-MB231 were treated with Heparan sulfate (20 µg/mL) for 24 h and detected by Fluorescence microscopy. RESULTS: Cell spreading was enhanced and the average spreading cell area approximately doubled in the presence of Heparan sulfate. Exogenous Heparan sulfate blocked cell invasion and matrix degradation. [1] |
| In vivo | METHODS: To assay anti-tumor activity in vivo, Heparan sulfate (0.8 mg/kg) was administered to SCID-hu mice injected with bone marrow aspirates from myeloma patients twice daily, once intraperitoneally and once subcutaneously, for four weeks. RESULTS: Treatment with Heparan sulfate significantly inhibited primary tumor growth in a human severe combined immunodeficiency (SCID-hu) myeloma model. [2] |
| Molecular Weight | 593.47(monomer) |
| Formula | C12H19NO20S3 (monomer) |
| Cas No. | 9050-30-0 |
| Relative Density. | no data available |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO: Insoluble  H2O: 100 mg/mL, Sonication is recommended. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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