Hematoxylin and Eosin (H&E) staining is commonly employed to visualize tissue morphology in histopathological analysis. Hematoxylin functions by oxidation and subsequent complex formation with metal mordants (e.g., aluminum or iron salts), yielding positively charged dye complexes that selectively bind to negatively charged phosphates within cell nuclei, producing a blue or bluish-purple coloration. Eosin, an acidic dye with negatively charged ions, stains cytoplasmic components and extracellular matrix proteins pink or reddish through electrostatic interactions with positively charged amino groups. This method is extensively used for assessing cellular structures and tissue pathology in paraffin or frozen tissue sections, as well as cytological preparations. Provided solutions are ready-to-use working concentrations, compatible for direct staining protocols without further dilution.