Hamster Interleukin 6,IL-6 ELISA Kit
Referência CSB-E14304Ha-5x96T
Tamanho : 5x96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Interleukin 6,IL-6
Abbreviation
IL-6
Species
Mesocricetus auratus (Golden hamster)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
Recovery
| The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-104 | ||||
| EDTA plasma (n=4) | 91 | 85-95 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Interleukin 6 (IL-6) is a multifunctional cytokine that plays essential roles in immune responses, inflammation, and hematopoiesis. This pleiotropic protein regulates acute-phase responses, promotes B-cell differentiation and antibody production, and influences T-cell activation. IL-6 also functions in metabolic processes and tissue repair mechanisms. Dysregulated IL-6 expression is associated with various pathological conditions. This makes it an important biomarker for inflammatory diseases, autoimmune disorders, and certain malignancies. Its diverse biological activities have made IL-6 a...
Citations
- Q Liu,Microorganisms,2023
- W Mohammedsaeed,Sudan Journal of Medical Sciences,2023
- X Liu,Advanced science,2023
- Asmaa G. Saleh.et al,IOSR Journal of Agriculture and Veterinary Science,/
- Saleh AG.et al,J Vet Med Sci.,2017
- Chang CW. et al,BMC Complement Altern Med,2015
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