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> Goat Tri-iodothyronine(T3) ELISA KIT

Goat Tri-iodothyronine(T3) ELISA KIT

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Referência CSB-E13385G-24T

Tamanho : 24T

Marca : Cusabio

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Telefone : +1 850 650 7790

CSB-E13385G
Size 96T,5×96T,10×96T

Product Details

Target Name
Tri-iodothyronine(T3)
Abbreviation
T3
Species
Capra hircus (Goat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.5 ng/mL-8 ng/mL
Sensitivity
0.5 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 95    
Range % 90-103    
1:2 Average % 95    
Range % 85-102    
1:4 Average % 93    
Range % 90-97    
1:8 Average % 94    
Range % 88-99    
Recovery
The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 86-102    
EDTA plasma (n=4) 96 92-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average 　    
8 0.166 0.168 0.167 　    
4 0.281 0.288 0.285 　    
2 0.558 0.553 0.556 　    
1 1.262 1.247 1.255 　    
0.5 2.105 2.138 2.122 　    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Goat Tri-iodothyronine (T3) ELISA Kit was designed for the quantitative measurement of Goat Tri-iodothyronine (T3) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-8 ng/mL and the sensitivity is 0.5 ng/mL.

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■ Q&A
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13385G,Goat Tri-iodothyronine(T3) ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
the standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then...