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> Goat Interleukin 10,IL-10 ELISA Kit

Goat Interleukin 10,IL-10 ELISA Kit

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Referência CSB-E09809g-24T

Tamanho : 24T

Marca : Cusabio

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CSB-E09809g
Size 96T,5×96T,10×96T

Product Details

Target Name
Interleukin 10,IL-10
Abbreviation
Uniprot No.
Species
Capra hircus (Goat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 82  
Range % 80-85  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 89  
Range % 84-93  
1:8 Average % 107  
Range % 103-111  
Recovery
The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 85 80-90  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.725 2.768 2.747 2.615  
1000 2.059 2.000 2.030 1.898  
500 1.321 1.368 1.345 1.213  
250 0.741 0.758 0.750 0.618  
125 0.455 0.432 0.444 0.312  
62.5 0.307 0.301 0.304 0.172  
31.25 0.228 0.226 0.227 0.095  
0 0.131 0.133 0.132    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Goat IL10 ELISA Kit was designed for the quantitative measurement of Goat IL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Citations

  • BSA Al Sulivany, M Asif, RA Asim, AM Mohammad,Tropical Animal Health and Production,2026
  • Z Shen,Vaccines,2023
  • X Lv,Food Science & Nutrition,2022
  • Yan F, et al,Viruses,2019
  • Jinzhen Jiao.et al,J Agric Food Chem,2018
  • Y. Ajith.et al,Veterinary Parasitology,2017
  • Ajith Y.et al,Parasite Immunol,2017

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