Goat Immunoglobulin A(IgA) ELISA Kit
Referência CSB-E13135G-5x96T
Tamanho : 5x96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Immunoglobulin A(IgA)
Abbreviation
IgA
Species
Capra hircus (Goat)
Sample Types
serum, plasma
Detection Range
29 ng/mL-7500 ng/mL
Sensitivity
29 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5000 | Average % | 94 | ||||
| Range % | 90-103 | |||||
| 1:10000 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:20000 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:40000 | Average % | 92 | ||||
| Range % | 85-103 | |||||
Recovery
| The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-100 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Goat IgA ELISA Kit was designed for the quantitative measurement of Goat IgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 29 ng/mL-7500 ng/mL and the sensitivity is 29 ng/mL.
Citations
- X Lv,Food Science & Nutrition,2022
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