Goat Anti-ZO-1 Antibody
Referência AF2206a
Tamanho : 100ug
Marca : Abcepta
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Goat Anti-ZO-1 Antibody
Peptide-affinity purified goat antibody
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AF2206a (0.3 µg/ml) staining of Human Cerebral Cortex lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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EB09206 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showingcellular junction staining. The nuclear stain is DA
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EB09206 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane, cytoplasmic and nuclear staining. The nu
Application 
| IF, Pep-ELISA |
|---|---|
| Primary Accession | Q07157 |
| Other Accession | NP_783297, 7082 |
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 195459 Da |
| Gene ID | 7082 |
|---|---|
| Other Names | Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1 |
| Dilution | IF~~1:50~200 Pep-ELISA~~N/A |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-ZO-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TJP1 (HGNC:11827) |
|---|---|
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity). |
| Cellular Location | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Cell projection, podosome. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). Forms a condensed tight junction-linked belt of protein during junction formation which becomes anchored to the apical cell membrane via interaction with PATJ (By similarity). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113). Colocalizes with SPEF1 at sites of cell- cell contact in intestinal epithelial cells (PubMed:31473225) {ECO:0000250|UniProtKB:O97758, ECO:0000269|PubMed:20930113, ECO:0000269|PubMed:31473225, ECO:0000269|PubMed:7798316} |
| Tissue Location | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules |
Research Areas
- Primary Antibodies
- Antibody Collections
- Goat Antibodies
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